Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

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Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

INTRODUCTION ........................................................ 291 General ............... ......................................... 291 Cell Waill Structure and Synthesis ....................... ........................ 292 Identification of the Target of Penicillin Action .......... ........................ 293 PENICILLIN-BINDING COMPONENTS .............. .......................... 296...

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The active centres in penicillin-sensitive enzymes.

The interaction between beta-lactam antibiotics and the penicillin-sensitive enzymes is a multiple-step process. Binding of the beta-lactam ring of the penam (or 3-cepham) nucleus occurs at binding site no. 1. Interaction between the N-14 substituent of the bound molecule and binding site no. 2 induces changes in binding site no. 1. In turn, the catalytic site thus created increases the chemica...

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Bacterial Resistance to Penicillin G by Decreased Affinity of Penicillin-Binding Proteins: A Mathematical Model

Streptococcus pneumoniae and Neisseria meningitidis have very similar mechanisms of resistance to penicillin G. Although penicillin resistance is now common in S. pneumoniae, it is still rare in N. meningitidis. Using a mathematical model, we studied determinants of this difference and attempted to anticipate trends in meningococcal resistance to penicillin G. The model predicted that pneumococ...

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Interaction of (2,3)-methylenepenams with penicillin-binding proteins.

A series of (2,3)-methylenepenams were examined with respect to binding to essential penicillin-binding proteins (PBPs) in Escherichia coli and Staphylococcus aureus. The compounds were also examined with respect to their interaction with Streptomyces strain R61 DD-carboxypeptidase. The alpha isomer of (2,3)-methylene penicillin G bound to PBP 3 of E. coli and other enterobacteria at 0.1 to 10 ...

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The formation of functional penicillin-binding proteins.

A method was developed which permitted determination of the [14C]benzylpenicillin and [14C]Cephapirin binding capacity of rapidly growing Bacillus subtilis cells in liquid culture. Over the concentration range of the binding plateau (0.1 to 0.8 mug/ml), [14C]benzylpenicillin significantly inhibited formation of functional penicillin-binding proteins, but had comparatively little effect on total...

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ژورنال

عنوان ژورنال: Bacteriological Reviews

سال: 1974

ISSN: 0005-3678

DOI: 10.1128/mmbr.38.3.291-335.1974